ANTIBACTERIAL TEST REPORT

2O and similar batch prepared and treated with Mica Miracle solution2O 􀂱 Hardy Diagnostics3 CFU/pellet􀂱 Petrifilm 􀂱 E. coli/Coliform

11. Incubate 24 hours at 35°± 1°C

12. Add o.1 ml of Mica Miracle solution to spiked sample with E. coli ATCC

51813 at 6.1 x 10

 

Escherichia coli Present Absent

CONCLUSION

The Mica Miracle solution prepared at 1:1000 effectively kills E. coli ATCC 51813

CONCLUSION

The Mica Miracle solution prepared at 1:1000 effectively kills E. coli ATCC 51813

present in the challenge water at about 6.1 x 10

Marco Almanza

Lab Manager3 CFU /ml.

Parameter Control

Samples

Mica Miracle

Solution

RESULTS

3 CFU/pellet

ANTIBACTERIAL TEST REPORT

Date: March 2, 2011

Standard Performed: Antibacterial Test

Method Performed: 3M Petrifilm

By: Marco Almanza

In-House Lab Test at Organic By Nature, Inc.

INTRODUCTION

Mica Miracle solution manufactured by Organic By Nature, Inc. was tested for

antibacterial properties. Control batch prepared with E. coli ATCC 51813 in

sterile DI H

at 1:1000 dilution. Control and test batches were tested with 3M Petrifilm E.

coli/Coliform plates.

MATERIALS

Mica Miracle concentrated solution

Sterile DI H

Microbiologics EZ-FPC E. coli ATCC 51813 at 6.1x10

3M

Incubator 35°± 1°C

PROCEDURE

1. Remove the vials from the refrigerator and allow them to warm to room

temperature.

2. Place the sterilized water containers in the incubator at 35°± 1°C for

approximately 30 minutes.

3. After the 30-minute warming period, remove one sterilized water container

from the incubator.

4. Carefully open the sterilized water container.

5. Open the appropriate bacteria sample vial and aseptically transfer the

gelatin tablet into the sterilized water container and labeled accordingly.

6. Reseal the sterilized water container that now contains the bacteria

sample.

7. Aseptically add 1 E. coli pellet to sterile DI water and control.

8. Place the inoculated samples into the incubator at 35°± 1°C for an

additional 30 minutes to dissolve the samples.

9. After the 30-minute dissolution period, gently shake each inoculated

sample to ensure that the gelatin tablet has completely dissolved.

10. Inoculate 3M Petrifilm with 1 ml of spiked solution and control